CAV2 Knockout cell line(BxPC-3)

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CAV2 Knockout cell line(BxPC-3) Order

CAV2 Knockout cell line(BxPC-3)
Catalog#
YKO-HJ051
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CAV2 Knockout cell line(BxPC-3)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CAV2 Knockout cell line (BxPC-3)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
CAV2
Gene ID
858
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 1 (based on transcript CAV2-201)
Validation: PCR+Sanger Sequencing
CAV2 Knockout cell line(BxPC-3) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:BxPC-3		 STR Info (Cell bank)
Cell Line:BX-PC3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11111111
D7S820 10131013
D16S539 911911
VWA 14181418
TH01 9999
AMEL XXXX
TPOX 8888
CSF1PO 13131313
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CAV2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BxPC-3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CAV2 gene knockout CAV2 Knockout cell line (BxPC-3) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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